Paper - Review

DOI: 10.1073/pnas.2017578118

Abstract

mRNAs → are localized & translated
← within neuronal dendrites & axons
→ to provide proteins → for 1⃣ remodeling 2⃣ maintaining growth (cones & synapse)

Whether (specific forms) ← of plasticity
→ regulate the dynamics & translation ← of individual mRNA species

❗: targeted → 3 individual synaptically localized mRNAs
← 1⃣ Camklla 2⃣ β-actin 3⃣ Psd95
❗: used molecular beacons → to track endogenous mRNA movements

Used reporters & CRISPR/Cas9 gene editing
→ to track mRNA translation ← in cultured neurons

∴ found → alterations ← in mRNA dynamics properties
← occurred during two forms of synaptic plasticity
∴ 1⃣ long-term potentiation 2⃣ depression

Changes ← in mRNA dynamics
← following either form of plasticity
→ results → in an enrichment of mRNA
← in the vicinity of dendritic spines

Both 1⃣ the reporters 2⃣ the tagging ← of endogenous proteins
→ revealed the transcript-specific stimulation of protein synthesis
← following 1⃣ cLTP 2⃣ mGluR-LTD

The plasticity-induced enrichment of mRNA ← near synapses
→ could be uncoupled ← from its translational status

The enrichment of mRNA
← in the proximity of spines
→ allows → for localized signaling pathways
→ to 1⃣ decode plasticity milieus 2⃣ stimulate a specific translational profiles
∴ A customized remodeling of the synaptic proteome

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