Paper - Review

DOI: 10.1126/SCIIMMUNOL.ABD5778

Abstract

TILs
← tumor-infiltrating lymphocytes
← especially CD8+ TILs
→ represent → a favorable prognostic factor
← in NSCLC

1⃣ the tissue origin 2⃣ regenerative capacities 3⃣ differentiation pathways
← of TIL sub-populations
→ remain poorly understood

❗: Investigate → the functional organization of TIL populations
← in primary NSCLC
∵ using → a combination of 1⃣ single-cell RNA 2⃣ T-cell receptor (TCR) sequencing

❗: identify → two CD8+ TIL sub-populations
→ expressing memory-like gene modules
∴ 1⃣ one is present ← in blood 2⃣ one ← in juxtatumor tissue

These tumor precursor populations
→ converge → through a unique transitional state
→ into terminally differentiated cells
← referred to as 1⃣ dysfunctional 2⃣ exhausted

Differentiation → is associated
← with TCR expansion
Transition
→ from precursor to late-differentiated states
→ correlates ← with intra-tumor T-cell cycling

❗: Provide → a coherent working model
→ for 1⃣ TIL origin 2⃣ ontogeny 3⃣ functional organization ← in primary NSCLC

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